ASSOCIATION OF THE SERUM IGG LEVEL AGAINST PERIODONTAL BACTERIA WITH PERIODONTAL STATUS AND SERUM LIPID LEVELS

Background and Objective

Several studies have reported that serum antibodies against periodontal pathogens are related not only to periodontal status but also to systemic conditions, such as glycemic control and serum lipids. Therefore, this study examined the associations of serum IgG antibodies against periodontal pathogens with periodontal status and serum lipid levels in community dwellers.

Materials and Methods

A community-based cross-sectional study of 409 subjects (140 men, 269 women) aged ≥ 40 years who had at least 5 teeth was conducted in Goto, Japan in 2009 and 2010.

Results

The serum IgG levels against Porphyromonas gingivalis were significantly associated with periodontal parameters and serum high-density lipoprotein cholesterol (HDL-C) in men. According to a multiple linear regression analysis adjusted for covariates, the serum IgG levels against P. gingivalis were inversely related to serum HDL-C (B = −0.1; P = 0.004).

Conclusion

This study suggests that an elevated immune response to the periodontal pathogen is independently cor-related with lower serum HDL-C level in community-dwelling men.

Periodontitis; Periodontal bacteria; Serum IgG antibody, HDL-cholesterol

1. Haffajee AD, Socransky SS. Microbiology of peri-odontal diseases: introduction. Periodontology 2000 2005;38:9–12.

2. Murayama Y, Nagai A, Okamura K, et al. Serum im-munoglobulin G antibody to periodontal bacteria. Adv Dent Res 1988;2(2):339–45.

3. Kudo C, Naruishi K, Maeda H, et al. Assessment of the plasma/serum IgG test to screen for periodontitis. J Dent Res 2012;91(12):1190–5.

4. Sugi N, Naruishi K, Kudo C, et al. Prognosis of peri-odontitis recurrence after intensive periodontal treatment using examination of serum IgG antibody titer against periodontal bacteria. J Clin Lab Anal 2011;25(1):25–32.

5. Saito T, Shimazaki Y, Sakamoto M. Obesity and peri-odontitis. N Engl J Med 1998;339(7):482–3.

6. Loe H. Periodontal disease. The sixth complication of diabetes mellitus. Diabet Care 1993;16(1):329–34.

7. Katz J, Flugelman MY, Goldberg A, et al. Association between periodontal pockets and elevated cholesterol and low density lipoprotein cholesterol levels. J Peri-odontol 2002;73(5):494–500.

8. Losche W, Karapetow F, Pohl A, et al. Plasma lipid and blood glucose levels in patients with destructive peri-odontal disease. J Clin Periodontol 2000;27(8):537–41.

9. Moore KJ, Tabas I. Macrophages in the pathogenesis of atherosclerosis. Cell 2011;145(3):341–55.

10. Gibson FC, 3rd, Hong C, Chou HH, et al. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation 2004;109(22):2801–6.

11. Zaremba M, Gorska R, Suwalski P, et al. Evaluation of the incidence of periodontitis-associated bacteria in the atherosclerotic plaque of coronary blood vessels. J Periodontol 2007;78(2):322–7.

12. MacAulay MA, Jacklyn CL, Mathers JM, et al. Con-tinuous-flow enzymatic determination of total serum cholesterol and method standardization with CDC-calibrated pooled sera. Clin Chem 1980;26(7):896–902.

13. Sullivan DR, Kruijswijk Z, West CE, et al. Determina-tion of serum triglycerides by an accurate enzymatic method not affected by free glycerol. Clin Chem 1985;31(7):1227–8.

14. Okada M, Matsui H, Ito Y, et al. Direct measurement of HDL cholesterol: method eliminating apolipoprotein 

E- rich particles. J Clin Lab Anal 2001;15(4):223–9.

15. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499–502.

16. Fritsche HA, Dee JW, Adams-Park HR. Enzymatic end-point analysis of glucose with the hexokinase method and the Union Carbide fast centrifugal analyzer. Clin Biochem 1975;8(4):240–6.

17. Kopp HP, Festa A, Hopmeier P, et al. [Evaluation of a new method for determining glycated hemoglobin with monoclonal antibodies (DCA 2000)]. Wiener klinische Wochenschrift 1996;108(1):16–9.

18. Brown LJ, Brunelle JA, Kingman A. Periodontal status in the United States, 1988-1991: prevalence, extent, and demographic variation. J Dent Res 1996;75 Spec No:672–83.

19. Hayashida H, Saito T, Kawasaki K, et al. Association of periodontitis with carotid artery intima-media thickness and arterial stiffness in community-dwelling people in Japan: the Nagasaki Islands study. Atherosclerosis 2013;229(1):186–91.

20. Cekici A, Kantarci A, Hasturk H, et al. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000 2014;64(1):57–80.

21. Nakagawa S, Machida Y, Nakagawa T, et al. Infection by Porphyromonas gingivalis and Actinobacillus actino-mycetemcomitans, and antibody responses at different ages in humans. J Periodont Res 1994;29(1):9–16.

22. Gordon DJ, Probstfield JL, Garrison RJ, et al. High-density lipoprotein cholesterol and cardiovascular disease. Four prospective American studies. Circulation 1989;79(1):8–15.

23. Khovidhunkit W, Kim MS, Memon RA, et al. Effects of infection and inflammation on lipid and lipoprotein metabolism: mechanisms and consequences to the host. J Lipid Res 2004;45(7):1169–96.

24. Post WS, Budoff M, Kingsley L, et al. Associations between HIV infection and subclinical coronary ath-erosclerosis. Ann Intern Med 2014;160(7):458–67.

25. Feingold KR, Grunfeld C. The acute phase response inhibits reverse cholesterol transport. J Lipid Res 2010;51(4):682–4.

26. Fisher EA, Feig JE, Hewing B, et al. High-density lipoprotein function, dysfunction, and reverse cho-lesterol transport. Arterioscler Thromb Vasc Biol 2012;32(12):2813–20.

27. Nizam N, Gumus P, Pitkanen J, et al. Serum and salivary matrix metalloproteinases, neutrophil elastase, myeloperoxidase in patients with chronic or aggressive periodontitis. Inflammation 2014;37(5):1771–8.

28. Wakatsuki A, Ikenoue N, Sagara Y. Effects of estrogen on susceptibility to oxidation of low-density and high-density lipoprotein in postmenopausal women. Maturitas 1998;28(3):229–34.