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Original Research

Open Access

ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro

  • Chuanqian Huang1
  • Changdan Chen2
  • Fangjing Zheng1
  • Xiaoxiao Ni1
  • Jianfang Lin1
  • Wenjuan Wu1
  • Xiaolan Lai3

1Department of Medical Oncology and Radiotherapy, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

2Department of Gastroenterology, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

3Department of Hematology and Rheumatism, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

DOI: 10.31083/jomh.2021.072 Vol.17,Issue 4,September 2021 pp.287-294

Submitted: 06 April 2021 Accepted: 08 June 2021

Published: 30 September 2021

*Corresponding Author(s): Xiaolan Lai E-mail: drxiaolan@163.com

Abstract

Background and objective: Activating transcription factor 3 (ATF3) plays a crucial role in regulating tumor development depending on the cellular context or cancer cell type. However, the effect of ATF3 on stem cells-like features in colorectal cancer (CRC) has yet to be elaborated.

Methods: In this study, we overexpressed ATF3 in SW620 CRC cells to investigate its effects on stem cells-like features.

Results: Our results indicated that overexpressing ATF3 inhibited the proliferation, invasion, migration, and sphere formation capacity of SW620 CRC cells. ATF3 overexpression also decreased the size of tumorspheres and reduced expression of the cancer stem cell markers CD44 and CD133 in SW620 cells.

Conclusion: In summary, our study revealed that ATF3 suppresses CRC growth and stem cells-like features. ATF3 is considered a potential target in CRC therapy.

Keywords

ATF3; Migration; Invasion; Cancer stem cells

Cite and Share

Chuanqian Huang,Changdan Chen,Fangjing Zheng,Xiaoxiao Ni,Jianfang Lin,Wenjuan Wu,Xiaolan Lai. ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro. Journal of Men's Health. 2021. 17(4);287-294.

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