The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA

Main Article Content

Saad Alshahrani
Gulfam Ahmad
Haroon Latif Khan
Ahmet Ayaz
Ali Hassan A. Ali

Keywords

Semen Analysis; DNA fragmentation; male infertility; sperm abnormalities.

Abstract

Abstract


Background and Objective: The aim of present study was to identify the impact of defective standard sperm parameters individually and in combination on DNA damage in a large cohort of infertile men.


Material and Methods: Retrospective analysis of semen characteristics was conducted on 436 patients. DNA fragmentation analysis was performed by using the terminal deoxynucleotidyl transferase (TdT)-mediated fluorescein-dUTP nick end labeling (TUNEL) assay. Sperm parameters were arranged into different categories such as normospermia asthenospermic, teratospermic, asthenoteratospermic and oligoasthenoteratospermic. Graph Pad Prism version 7 software was used for data analysis.


Results: Our results suggest that the mean percentage of DNA damage was proportionally higher than the the semen abnormalities. Sperm with three abnormal parameters showed significantly higher DNA damage suggesting that sperm having more than two abnormalities are more likely to have higher DNA damage.


Conclusion: Sperm motility had significant correlation and is supposed to be a predictor for these tests, while morphology was the second standard sperm parameter inversely correlated with sperm DNA damage. Patients demonstrating low levels of leukocytospermia should be adviced sperm DNA testing before ART treatment. However, there is a clear need for more research studies to further address these issues.

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