The IMPACT OF SINGLE AND MULTIPLE SPERM ABNORMALITIES AND LOW-LEVEL LEUKOCYTOSPERMIA ON SPERM DNA

Main Article Content

Saad Alshahrani
Gulfam Ahmad
Haroon Latif Khan
Ahmet Ayaz
Ali Hassan A. Ali

Keywords

semen analysis, DNA fragmentation, male infertility, sperm abnormalities

Abstract


Background and objective
The aim of the present study was to identify the impact of defective standard sperm parameters individually and in combination on DNA damage in a large cohort of infertile men.
Material and methods
Retrospective analysis of semen characteristics was conducted on 436 patients. DNA fragmentation analysis was performed by using the terminal deoxynucleotidyl transferase (TdT)-mediated fluoresce-in-dUTP nick end labeling (TUNEL) assay. Sperm parameters were arranged into different categories such as normospermia asthenospermic, teratospermic, asthenoteratospermic, and oligoasthenoteratospermic. GraphPad Prism version 7 software was used for data analysis.
Results
Our results suggest that the mean percentage of DNA damage was proportionally higher than the semen abnormalities. Sperm with 3 abnormal parameters showed significantly higher DNA damage, suggesting that sperm having more than 2 abnormalities are more likely to have higher DNA damage.
Conclusion
Sperm motility had significant correlation and is supposed to be a predictor for these tests, while morphology was the second standard sperm parameter inversely correlated with sperm DNA damage. Patients demonstrating low levels of leukocytospermia should be advised sperm DNA testing before assisted reproductive technology treatment. However, there is a clear need for more research studies to further address these issues.
 

 

Downloads

Download data is not yet available.
Abstract 162 | pdf Downloads 90 HTML Downloads 13 xml Downloads 0

References

1. Alshahrani S, Aldossari K, Al-Zahrani J, Gabr AH, Henkel R, Ahmad G. Interpretation of semen analysis using WHO 1999 and WHO 2010 reference values: abnormal becoming normal. Andrologia 2018 Mar;50(2):e12838. Available at: https://www.ncbi.nlm.nih.gov/pubmed/28771785
2. Agarwal A, Majzoub A, Esteves SC, Ko E, Ramasamy R, Zini A. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Transl Androl Urol 2016 Dec;5(6):935. https://doi.org/10.21037/tau.2016.10.03
3. Cooper TG, Noonan E, Von Eckardstein S, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010 Jan 1;16(3):231–45. Available at: https://www.ncbi.nlm.nih.gov/pubmed/19934213
4. Al Omrani B, Al Eisa N, Javed M, Al Ghedan M, Al Matrafi H, Al Sufyan H. Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome. Reprod Biol Endocrinol 2018 Dec;16(1):49. https://doi.org/10.1186/s12958-018-0369-3
5. Oehninger S. Clinical and laboratory management of male infertility: an opinion on its current status. J Androl 2000 Nov 12;21(6):814–21. https://doi.org/10.1002/j.1939-4640.2000.tb03411.x
6. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update. 2003 Jul 1;9(4):331–45. Available at: https://www.ncbi.nlm.nih.gov/pubmed/12926527
7. Lewis SE, Aitken RJ, Conner SJ, et al. The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Reprod Biomed Online 2013 Oct 1;27(4):325–37. https://doi.org/10.1016/j.rbmo.2013.06.014
8. Hammoud AO, Gibson M, Peterson CM, Meikle AW, Carrell DT. Impact of male obesity on infertility: a critical review of the current literature. Fertil Steril 2008 Oct 1;90(4):897–904. https://doi.org/10.1016/j.fertnstert.2008.08.026
9. Saleh RA, Agarwal A, Nada EA, et al. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril 2003 Jun 1;79:1597–605. https://doi.org/10.1016/S0015-0282(03)00337-6
10. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 2002 Jan 2;23(1):25–43. https://doi.org/10.1002/j.1939-4640.2002.tb02599.x
11. Spanò M, Bonde JP, Hjøllund HI, et al. Sperm chromatin damage impairs human fertility. Fertil Steril 2000 Jan 1;73(1):43–50. https://doi.org/10.1016/S0015-0282(99)00462-8
12. Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online 2006 Jan 1;12(4):466–72. https://doi.org/10.1016/S1472-6483(10)62000-7
13. Esteves SC, Sharma RK, Gosálvez J, Agarwal A. A translational medicine appraisal of specialized andrology testing in unexplained male infertility. Int Urol Nephrol 2014 Jun 1;46(6):1037–52. https://doi.org/10.1007/s11255-014-0715-0
14. Ahmad G, Moinard N, Esquerré-Lamare C, Mieusset R, Bujan L. Mild induced testicular and epididymal hyperthermia alters sperm chromatin integrity in men. Fertil Steril 2012 Mar 1;97(3):546–53. https://doi.org/10.1016/j.fertnstert.2011.12.025
15. Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: the influence of stress and quality of life on male fertility. Reprod Biol Endocrinol 2018 Dec;16(1):115. https://doi.org/10.1186/s12958-018-0436-9
16. Rehman S, Usman Z, Rehman S, et al. Endocrine disrupting chemicals and impact on male reproductive health. Transl Androl Urol 2018 Jun;7(3):490–503. https://doi.org/10.21037/tau.2018.05.17
17. Simon L, Emery BR, Carrell DT. Diagnosis and impact of sperm DNA alterations in assisted reproduction. Best Pract Res Clin Obstet Gynaecol 2017 Oct 1;44:38–56. https://doi.org/10.1016/j.bpobgyn.2017.07.003
18. Simon L, Zini A, Dyachenko A, Ciampi A, Carrell DT. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl 2017 Jan;19(1):80. https://doi.org/10.4103/1008-682X.182822
19. Giwercman A, Richthoff J, Hjøllund H, et al. Correlation between sperm motility and sperm chromatin structure assay parameters. Fertil Steril 2003 Dec 1;80(6):1404–12 https://doi.org/10.1016/S0015-0282(03)02212-X
20. Das M, Al-Hathal N, San-Gabriel M, et al. High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age. J Assist Reprod Genet 2013 Jun 1;30(6):843–8. https://doi.org/10.1007/s10815-013-0015-0
21. Erenpreiss J, Elzanaty S, Giwercman A. Sperm DNA damage in men from infertile couples. Asian J Androl 2008 Sep;10(5):786–90. https://doi.org/10.1111/j.1745-7262.2008.00417.x
22. Moskovtsev SI, Willis J, White J, Mullen JB. Sperm DNA damage: correlation to severity of semen abnormalities. Urology 2009. Oct 1;74(4):789–93. https://doi.org/10.1016/j.urology.2009.05.043
23. Agarwal A, Mulgund A, Alshahrani S, et al. Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Reprod Biol Endocrinol 2014 Dec;12(1):126. https://doi.org/10.1186/1477-7827-12-126
24. Ahmad G, Agarwal A, Esteves SC, et al. Ascorbic acid reduces redox potential in human spermatozoa subjected to heat-induced oxidative stress. Andrologia 2017 Dec;49(10):e12773. https://doi.org/10.1111/and.12773