RT  - Journal of Men's Health
ID  - 10.22374/jomh.v15i2.144
T1  - IMPACT OF SINGLE AND MULTIPLE SPERM ABNORMALITIES AND LOW-LEVEL LEUKOCYTOSPERMIA ON SPERM DNA
A1  - Saad Alshahrani
A1  - Gulfam Ahmad
A1  - Haroon Latif Khan
A1  - Ahmet Ayaz
A1  - Ali Hassan A. Ali
K1  - semen analysis
K1  - DNA fragmentation
K1  - male infertility
K1  - sperm abnormalities
YR  - 2019
SP  - 58
AB  - <p class="ql-align-justify"><strong>Background and objective</strong></p><p class="ql-align-justify">The aim of the present study was to identify the impact of defective standard sperm parameters indi-vidually and in combination on DNA damage in a large cohort of infertile men.</p><p class="ql-align-justify"><strong>Material and methods</strong></p><p class="ql-align-justify">Retrospective analysis of semen characteristics was conducted on 436 patients. DNA fragmentation analysis was performed by using the terminal deoxynucleotidyl transferase (TdT)-mediated fluoresce-in-dUTP nick end labeling (TUNEL) assay. Sperm parameters were arranged into different categories such as normospermia asthenospermic, teratospermic, asthenoteratospermic, and oligoasthenoter-atospermic. GraphPad Prism version 7 software was used for data analysis.</p><p class="ql-align-justify"><strong>Results</strong></p><p class="ql-align-justify">Our results suggest that the mean percentage of DNA damage was proportionally higher than the semen abnormalities. Sperm with 3 abnormal parameters showed significantly higher DNA damage, suggesting that sperm having more than 2 abnormalities are more likely to have higher DNA damage.</p><p class="ql-align-justify"><strong>Conclusion</strong></p><p class="ql-align-justify">Sperm motility had significant correlation and is supposed to be a predictor for these tests, while mor-phology was the second standard sperm parameter inversely correlated with sperm DNA damage. Patients demonstrating low levels of leukocytospermia should be advised sperm DNA testing before assisted reproductive technology treatment. However, there is a clear need for more research studies to further address these issues.</p>