Effects of Exercise Intensity on Alcohol Dehydrogenase Gene Expression in the Rat Large Intestine

Exercise and ADH Gene Expression

Background and Objective

The aim of this study was to examine the relationship between the intensity of treadmill exercise and the expression of alcohol dehydrogenase (ADH) in the large intestine.

Materials and Methods

Thirty Sprague-Dawley male rats were randomly assigned to a control group (CON; no exercise), low-intensity exercise group (LIG; 30-min exercise at 8 m/min 5 times a week for 4 weeks), and high-intensity exercise group (HIG; 30-min exercise at 28 m/min 5 times a week for 4 weeks).

Results

Microarray analysis was conducted to evaluate ADH gene expression levels in large intestinal tissue. Sig-nificant changes in the expression of four ADH genes (Adh1, Adh4, Adh6a, and Adh7) were observed in relation to exercise intensity. In addition, pooled samples in the exercise groups showed decreased expres-sion levels of these four genes compared with those of the control group. These findings were confirmed by reverse transcription-polymerase chain reaction. In addition, differences were detected with respect to exercise intensity; Adh1, Adh4, and Adh6a levels were significantly decreased in the LIG compared with those in the HIG, whereas Adh7 expression showed an opposite trend.

Conclusion

In conclusion, this study suggested that regular exercise decreases the incidence of alcohol-related disease by suppressing ADH production in the digestive system.

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