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Original Research

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COMPARATIVE STUDY BETWEEN CLASSICAL AND MOLECULAR METHODS IN DIAGNOSIS OF LEISHMANIA SPECIES IN IRAQ

  • Magda A. Ali1,2
  • Ali Khamesipour1
  • Abdulsadah A. Rahi3
  • Mehdi Mohebali4,5
  • Amir Ahmad Akhavan6

1Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Science (TUMS-IC), Tehran, Iran

2Department of Microbiology, College of Medicine, Wasit University, Kut, Iraq

3Department of Biology, College of Science, Wasit University, Kut, Iraq

4Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences,Tehran, Iran

5Center for Research of Endemic Parasites of Iran (CREPI),Tehran University of Medical Sciences, Tehran, Iran

6Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: 10.22374/1875-6859.14.3.10 Vol.14,Issue 3,June 2018 pp.69-76

Published: 07 June 2018

*Corresponding Author(s): Ali Khamesipour E-mail: ali.khamesipour@gmail.com

Abstract

Background and Objective

Cutaneous leishmaniasis (CL) remains a serious public health concern in some parts of Iraq. The aims of this study to diagnose the Leishmania sp. causative agent of CL in some parts of Iraq, by different parasito-logical, cultural, and molecular methods. It was carried during the period October 2014 to February 2015.

Materials and Methods

One-hundred sixty-one skin samples were examined by direct Giemsa-smeared culture on NNN medium and Nested – PCR methods in different age groups.

Results

The results of our study showed that 110 (68.3%) gave positives by Giemsa-smeared, 104 (64.6%) culture and 67 (100%) by Nested – PCR. Our results appeared that there was slight gender predilection; CL cases were more frequent in males (62.1%) than females (37.9%). Also, the type of infection showed that disease was in wet type 133(82.6%) more than in dry type 28 (17.4%). It was found that 98 (60.9%) of CL cases had previous contact with rodents while 63(39.1%) of cases had not.

Conclusions

CL disease is endemic in many parts of Iraq with high incidence and expanding to new foci that is consid-ered a public health threat which needs special attention. Women appeared to be better equipped than men to contain the infection and its clinical consequences, but the sex factor tended to lessen at higher levels of exposure.

Keywords

Cutaneous Leishmaniasis, Giemsa, Culture, Human, N-PCR

Cite and Share

Magda A. Ali,Ali Khamesipour,Abdulsadah A. Rahi,Mehdi Mohebali,Amir Ahmad Akhavan. COMPARATIVE STUDY BETWEEN CLASSICAL AND MOLECULAR METHODS IN DIAGNOSIS OF LEISHMANIA SPECIES IN IRAQ. Journal of Men's Health. 2018. 14(3);69-76.

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