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Systematic review

Open Access

Meta-analysis of the relevance between sperm parameters and serum vitamin D levels in infertile men

  • Wenda Zou1,†
  • Ziwei Liu2,†
  • Juan Zhang1,*,

1Department of Reproductive Medicine Center, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, 412007 ZhuZhou, Hunan, China

2Department of Urology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, 412007 ZhuZhou, Hunan, China

DOI: 10.22514/jomh.2023.052 Vol.19,Issue 7,July 2023 pp.8-15

Submitted: 12 December 2022 Accepted: 22 February 2023

Published: 30 July 2023

*Corresponding Author(s): Juan Zhang E-mail:

† These authors contributed equally.


To systematically estimate the relevance between sperm parameters and serum vitamin D levels and the subsequent reproductive outcomes in men. PubMed, Web of Science and other databases were searched for studies that examined a possible correlation between serum vitamin D levels and sperm parameters and reproductive outcomes. The documents were screened according to the corresponding inclusion and exclusion criteria. Selection criteria included all published randomised and controlled trials and non-randomised studies that focus on vitamin D and the reproductive function of men The patients were classified into observation/intervention and control groups, the intervention group was given vitamin D supplementation and the control group was not. The meta-analysis was conducted using Stata 16.0 software. A meta-analysis of 11 clinical studies that enrolled a total of 1356 patients, revealed that the clinical pregnancy rate in the observation group was no statistically different in the control group. A meta-analysis of 8 studies demonstrated that there was no statistically difference in sperm concentration between the experimental group and the control group. A meta-analysis of 8 studies found that sperm progressive motility in the experimental group was greater than that in the control group by using a random-effects model. The mean concentration of vitamin D was significantly lower in the control group. Appropriate vitamin D supplement intervention in infertile men improves sperm progressive motility. Interpretation of these results should be done with caution due to the significant heterogeneity of the included studies. Furthermore, Well-conducted prospective cohort studies are needed to better explain the relationship between vitamin D and sperm parameters in infertile men.


Male infertility; Semen parameters; Serum vitamin D; Reproductive outcome

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Wenda Zou,Ziwei Liu,Juan Zhang. Meta-analysis of the relevance between sperm parameters and serum vitamin D levels in infertile men. Journal of Men's Health. 2023. 19(7);8-15.


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