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Original Research

Open Access

Effects of short-term high-intensity interval training on growth hormone, cortisol, and leptin levels

  • Muhammed Öniz1,*,
  • Nazmi Sarıtaş2
  • Meryem Şentürk3

1Institute of Health Sciences, Erciyes University, 38039 Kayseri, Turkey

2Faculty of Sport Sciences, Erciyes University, 38039 Kayseri, Turkey

3Department of Biochemistry, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri, Turkey

DOI: 10.22514/jomh.2023.124

Submitted: 30 August 2023 Accepted: 13 November 2023

Online publish date: 14 December 2023

*Corresponding Author(s): Muhammed Öniz E-mail:


Exercise is known to be very effective for increasing hormone levels; however, the effects of high-intensity interval training (HIIT) protocols are uncertain. The study aimed to examine the effect of HIIT on growth hormone (GH), cortisol, leptin levels and anaerobic capacity (AC) with the intention of contributing to the development of exercise protocols better adapted to athletes’ training programs and optimized for hormone regulation. In the study, participants underwent a 2-week HIIT protocol. They were randomly divided into two groups (n = 10 for HIIT and n = 10 for the control) in a double-blind manner. Twenty healthy male athletes aged 18–30 years were subjected to a short-term HIIT exercise on a bicycle ergometer. The blood and Wingate AC data collected in the first and last sessions of the protocol were analyzed, and the GH, cortisol, leptin and AC levels were examined. Based on the results of the analysis, statistically significant differences were observed in GH (p < 0.05), cortisol (p < 0.05), leptin (p < 0.001) and AC (p < 0.01) levels between the initial and final tests among the participants. However, while there were no statistically significant differences between the groups in GH (p = 0.088), cortisol (p = 0.905), leptin (p = 0.262) and AC (p = 0.06) levels. The study’s findings indicate that the short-term HIIT protocol caused significant differences in GH, leptin, cortisol and WAnT AC levels within the group depending on the time main effect. These results suggest that HIIT is efficient for biochemical changes and performance. However, no significant differences were found between the groups. Results suggest that HIIT protocols may elicit different responses between individuals and that time modulates these effects. It is recommended that future studies should be conducted with more participants and longer HIIT protocols.


Anaerobic capacity; Cortisol; Exercise; High-intensity interval training; Hormones; Leptin; Wingate test

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Muhammed Öniz,Nazmi Sarıtaş,Meryem Şentürk. Effects of short-term high-intensity interval training on growth hormone, cortisol, and leptin levels. Journal of Men's Health. 2024.doi:10.22514/jomh.2023.124.


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