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Comparison of plasma amino acid concentrations after whey protein hydrolysate intake in young men at rest and post-resistance exercise

  • Sunghwan Kyun1
  • Jeehee Choi1
  • Deunsol Hwang1
  • Inkwon Jang1
  • Sung-Woo Kim1,2
  • Hun-Young Park1,2
  • Kiwon Lim1,2,3
  • Jisu Kim1,2,*,

1Department of Sports Medicine and Science in Graduated School, Konkuk University, 05029 Seoul, Republic of Korea

2Physical Activity and Performance Institute (PAPI), Konkuk University, 05029 Seoul, Republic of Korea

3Department of Physical Education, Konkuk University, 05029 Seoul, Republic of Korea

DOI: 10.22514/jomh.2023.129 Vol.19,Issue 12,December 2023 pp.51-59

Submitted: 24 August 2023 Accepted: 27 September 2023

Published: 30 December 2023

*Corresponding Author(s): Jisu Kim E-mail:


Few studies have compared the differences between protein (whey protein hydrolysate (WPH) or whey protein concentrate (WPC)) intake conditions (at rest or post-resistance exercise). Therefore, the purpose of this study was to investigate the plasma amino acid levels after intake of WPH compared with WPC intake in young men at rest and post-resistance exercise. We also aimed to compare the differences in plasma amino acid levels after WPH intake with or without resistance exercise. Fifteen young men were recruited for this crossover study, where each participant took three supplements (placebo, WPH and WPC) once each at rest and post-resistance exercise over 9 weeks. Blood samples were collected at nine (0, 15, 30, 45, 60, 90, 120, 180, 240 min) and ten (pre, 0, 15, 30, 45, 60, 90, 120, 180, 240 min) time points, at rest and post-resistance exercise, respectively. Plasma amino acids (total amino acids, essential amino acids, branched amino acids and leucine) were measured. WPH intake resulted in faster bioavailability (approximately 15 min) and higher plasma amino acid concentrations in all plasma amino acids, regardless of the condition. In addition, WPH bioavailability tended to have similar peaks concentrations at rest and post-resistance exercise, whereas post-resistance exercise of WPC decreased sharply after 30 min and maintained a lower concentration than normal after 60 min. In conclusion, the results that WPH intake initially increased the concentration of plasma amino acids than WPC suggests that WPH is absorbed into the blood faster and may be more effective in increasing the rate of muscle protein synthesis. Moreover, although the intake of WPH after resistance exercise does not upregulate the peak concentrations of plasma amino acids, the synthesis of skeletal muscle can be increased through a rapid supply to tissues that require amino acids.


Protein intake; Whey protein hydrolysate; Resistance exercise; Plasma amino acid; Aminoacidemia

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Sunghwan Kyun,Jeehee Choi,Deunsol Hwang,Inkwon Jang,Sung-Woo Kim,Hun-Young Park,Kiwon Lim,Jisu Kim. Comparison of plasma amino acid concentrations after whey protein hydrolysate intake in young men at rest and post-resistance exercise. Journal of Men's Health. 2023. 19(12);51-59.


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