Article Data

  • Views 342
  • Dowloads 138

Original Research

Open Access

Qingre Jiangni formula improves esophageal epithelial barrier function in gastroesophageal reflux rats through male hormones and the p38/MAPK pathway

  • Zhengyan Jiang1,†
  • Dianqiang Lu1,†
  • Zhiguang Sun2,*,
  • Wei Wen1,*,

1The Second Affiliated Hospital of Nanjing University of Chinese Medicine, 210000 Nanjing, Jiangsu, China

2Nanjing University of Chinese Medicine, 210000 Nanjing, Jiangsu, China

DOI: 10.22514/jomh.2024.013 Vol.20,Issue 1,January 2024 pp.99-106

Submitted: 12 September 2023 Accepted: 08 November 2023

Published: 30 January 2024

*Corresponding Author(s): Zhiguang Sun E-mail: zhiguangsun@njucm.edu.cn
*Corresponding Author(s): Wei Wen E-mail: Efy177@njucm.edu.cn

† These authors contributed equally.

Abstract

The incidence rate of gastroesophageal reflux is high. Qingre Jiangni Formula has shown good clinical efficacy. In this study, the therapeutic effect of this formula on inflammation was analyzed in the gastroesophageal reflux model. A rat model of gastroesophageal reflux was constructed using surgical methods. The successful construction of the rat model was confirmed through case testing. The model rats were treated with Qingre herbs, Jiangni herbs, and Qingre + Jiangni herbs for 2 weeks, followed by hematoxylin-eosin staining (HE), Western Blot (WB), enzyme linked immunosorbent assay (Elisa) and polymerase chain reaction (PCR) assays. SPSS 22.0 statistical software was used for analysis. The modeling of gastroesophageal reflux reduced the levels of testosterone and androsterol. The Qingre Jiangni formula helped to improve testosterone and androsterol to normal levels. Treatments in the Qingre group, Jiangni group, and Qingre + Jiangni group can all decrease inflammatory factor levels (interleukin IL-1B, IL-6, IL-8), and lead to a decreasing trend in p38, Claudin-4, Occludin and ZO_S. In addition, the three groups also showed decreased inflammation pathological levels compared with those in the model group (p < 0.01). Qingre Jiangni Formula is helpful for male hormones (testosterone and androsterol) and can decrease gastroesophageal reflux disease inflammation, thereby treating gastroesophageal reflux disease.


Keywords

Qingre Jiangni formula; Esophageal epithelial barrier; Gastroesophageal reflux disease; Testosterone; Androsterol


Cite and Share

Zhengyan Jiang,Dianqiang Lu,Zhiguang Sun,Wei Wen. Qingre Jiangni formula improves esophageal epithelial barrier function in gastroesophageal reflux rats through male hormones and the p38/MAPK pathway. Journal of Men's Health. 2024. 20(1);99-106.

References

[1] Nirwan JS, Hasan SS, Babar ZU, Conway BR, Ghori MU. Global prevalence and risk factors of gastro-oesophageal reflux disease (GORD): systematic review with meta-analysis. Scientific Reports. 2020; 10: 5814.

[2] Mermelstein J, Chait Mermelstein A, Chait MM. Proton pump inhibitor-refractory gastroesophageal reflux disease: challenges and solutions. Clinical and Experimental Gastroenterology. 2018; 11: 119–134.

[3] Harada K, Hanayama Y, Yasuda M, Hasegawa K, Obika M, Kataoka H, et al. Clinical relevance of low androgen to gastroesophageal reflux symptoms. Endocrine Journal. 2018; 65: 1039–1047.

[4] Cook MB, Wood SN, Cash BD, Young P, Acosta RD, Falk RT, et al. Association between circulating levels of sex steroid hormones and Barrett’s esophagus in men: a case-control analysis. Clinical Gastroenterology and Hepatology. 2015; 13: 673–682.

[5] Burotto M, Chiou VL, Lee JM, Kohn EC. The MAPK pathway across different malignancies: a new perspective. Cancer. 2014; 120: 3446–3456.

[6] Cuadrado A, Nebreda AR. Mechanisms and functions of p38 MAPK signalling. The Biochemical Journal. 2010; 429: 403–417.

[7] Falcicchia C, Tozzi F, Arancio O, Watterson DM, Origlia N. Involvement of p38 MAPK in synaptic function and dysfunction. International Journal of Molecular Sciences. 2020; 21: 5624.

[8] Blythe NM, Muraki K, Ludlow MJ, Stylianidis V, Gilbert HTJ, Evans EL, et al. Mechanically activated Piezo1 channels of cardiac fibroblasts stimulate p38 mitogen-activated protein kinase activity and interleukin-6 secretion. Journal of Biological Chemistry. 2019; 294: 17395–17408.

[9] Zhang X, Fan L, Wu J, Xu H, Leung WY, Fu K, et al. Macrophage p38α promotes nutritional steatohepatitis through M1 polarization. Journal of Hepatology. 2019; 71: 163–174.

[10] An Z, Li J, Yu J, Wang X, Gao H, Zhang W, et al. Neutrophil extracellular traps induced by IL-8 aggravate atherosclerosis via activation NF-κB signaling in macrophages. Cell Cycle. 2019; 18: 2928–2938.

[11] Coulthard LR, White DE, Jones DL, McDermott MF, Burchill SA. P38MAPK: stress responses from molecular mechanisms to therapeutics. Trends in Molecular Medicine. 2009; 15: 369–379.

[12] Sun J, Nan G. The mitogen-activated protein kinase (MAPK) signaling pathway as a discovery target in stroke. Journal of Molecular Neuroscience. 2016; 59: 90–98.

[13] Yeung YT, Aziz F, Guerrero-Castilla A, Arguelles S. Signaling pathways in inflammation and anti-inflammatory therapies. Current Pharmaceutical Design. 2018; 24: 1449–1484.

[14] Li C, Gao X, Gao X, Lv J, Bian X, Lv J, et al. Effects of medicine food Fructus Gardeniae on liver and kidney functions after oral administration to rats for 12 weeks. Journal of Food Biochemistry. 2021; 45: e13752.

[15] Pang X, Cai H, Zhao J, Sun P, Shi J, Zhang Y, et al. Efficacy of astragalus in the treatment of radiation-induced lung injury based on traditional Chinese medicine: a systematic review and meta-analysis of 25 RCTs. Medicine. 2022; 101: e30478.

[16] Zhang W, Xu J, Yu T, Chen Q. Effects of berberine and metformin on intestinal inflammation and gut microbiome composition in db/db mice. Biomedicine & Pharmacotherapy. 2019; 118: 109131.

[17] Zhang S, Xu P, Zhu Z, Zhou L, Li J, Zhou R, et al. Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine. Redox Biology. 2023; 62: 102704.

[18] Habtemariam S. Berberine pharmacology and the gut microbiota: a hidden therapeutic link. Pharmacological Research. 2020; 155: 104722.

[19] Dong Y, Fan H, Zhang Z, Jiang F, Li M, Zhou H, et al. Berberine ameliorates DSS-induced intestinal mucosal barrier dysfunction through microbiota-dependence and Wnt/β-catenin pathway. International Journal of Biological Sciences. 2022; 18: 1381–1397.

[20] Limanaqi F, Biagioni F, Busceti CL, Polzella M, Fabrizi C, Fornai F. Potential antidepressant effects of Scutellaria baicalensis, Hericium erinaceus and Rhodiola rosea. Antioxidants. 2020; 9: 234.

[21] Wan F, Wang M, Zhong R, Chen L, Han H, Liu L, et al. Supplementation with Chinese medicinal plant extracts from Lonicera hypoglauca and Scutellaria baicalensis mitigates colonic inflammation by regulating oxidative stress and gut microbiota in a colitis mouse model. Frontiers in cellular and infection microbiology. 2022; 11: 798052.

[22] Jiang M, Li H, Zhang Y, Yang Y, Lu R, Liu K, et al. Transitional basal cells at the squamous-columnar junction generate Barrett’s oesophagus. Nature. 2017; 550: 529–533.

[23] Mei K, Chen Z, Wang Q, Luo Y, Huang Y, Wang B, et al. The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease. Frontiers in Immunology. 2023; 13: 1067950.

[24] Jung HK, Tae CH, Song KH, Kang SJ, Park JK, Gong EJ, et al. 2020 Seoul consensus on the diagnosis and management of gastroesophageal reflux disease. Journal of Neurogastroenterology and Motility. 2021; 27: 453–481.

[25] Liu Y, Shi Y, Han R, Liu C, Qin X, Li P, et al. Signaling pathways of oxidative stress response: the potential therapeutic targets in gastric cancer. Frontiers in Immunology. 2023; 14: 1139589.

[26] Kim YS, Kim N, Kim GH. Sex and gender differences in gastroesophageal reflux disease. Journal of Neurogastroenterology and Motility. 2016; 22: 575–588.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Directory of Open Access Journals (DOAJ) DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online for everyone.

SCImago The SCImago Journal & Country Rank is a publicly available portal that includes the journals and country scientific indicators developed from the information contained in the Scopus® database (Elsevier B.V.)

Publication Forum - JUFO (Federation of Finnish Learned Societies) Publication Forum is a classification of publication channels created by the Finnish scientific community to support the quality assessment of academic research.

Scopus: CiteScore 0.7 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Norwegian Register for Scientific Journals, Series and Publishers Search for publication channels (journals, series and publishers) in the Norwegian Register for Scientific Journals, Series and Publishers to see if they are considered as scientific. (https://kanalregister.hkdir.no/publiseringskanaler/Forside).

Submission Turnaround Time

Conferences

Top