Article Data

  • Views 396
  • Dowloads 137

Original Research

Open Access

The use of network pharmacology to investigate the mechanism of action of Scutellaria barbata in the treatment of prostate cancer

  • Wenhui Zhu1,†
  • Lili Mao2,†
  • Ruijie Yang2,†
  • Shijun Tong1
  • Jing Zhai1
  • Guanxiong Ding1,*,

1Department of Urology, Huashan Hospital, Fudan University, 200040 Shanghai, China

2Department of Nursing, Huashan Hospital, Fudan University, 200040 Shanghai, China

DOI: 10.22514/jomh.2024.065 Vol.20,Issue 5,May 2024 pp.12-20

Submitted: 21 July 2023 Accepted: 26 September 2023

Published: 30 May 2024

*Corresponding Author(s): Guanxiong Ding E-mail: 10111220030@fudan.edu.cn

† These authors contributed equally.

Abstract

We aimed to identify the mechanism of action of the Scutellaria barbata in the treatment of prostate cancer. First, the main active components of Scutellaria barbata for the treatment of prostate cancer were predicted by network pharmacology. Disease-related targets were then retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP); GeneCards: The Human Gene Database; and the Online Catalog of Human Genes and Genetic Disorders (OMIM). Then, we identified intersecting genes between the components and targets. Cytoscape software was then used to construct a “Drug-Ingredient-Disease-Target” network. We also used the STRING platform to perform protein interaction analysis. R software was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Our analysis identified 212 potential targets for Scutellaria barbata in the treatment of prostate cancer; these were closely related to a number of specific pathways, including prostate cancer, the advanced glycation end products and their receptors (AGE-RAGE) signaling pathway in diabetic complications, lipids and atherosclerosis, hepatitis B, fluid shear stress and atherosclerosis, chemical carcinogenesis, receptor activation, Kaposi’s sarcoma-associated herpesvirus infection, and human cytomegalovirus infection. Furthermore, the treatment of prostate cancer with Scutellaria barbata may act via a range of biological processes, including responses to xenobiotic stimuli, lipopolysaccharides and metal ions. Several molecular functions may be involved, including DNA-binding transcription factor binding, RNA polymerase II-specific DNA-binding transcription factor binding, and nuclear receptor activity. Our research demonstrates the multi-component, multi-target, multi-pathway, and synergistic characteristics of Scutellaria barbata for the treatment of prostate cancer, and provides theoretical guidelines for further elucidating its mechanism of action.


Keywords

Prostate carcinomatosis; Scutellaria barbata; Network pharmacology; Antitumor


Cite and Share

Wenhui Zhu,Lili Mao,Ruijie Yang,Shijun Tong,Jing Zhai,Guanxiong Ding. The use of network pharmacology to investigate the mechanism of action of Scutellaria barbata in the treatment of prostate cancer. Journal of Men's Health. 2024. 20(5);12-20.

References

[1] Ghafouri-Fard S, Shabestari FA, Vaezi S, Abak A, Shoorei H, Karimi A, et al. Emerging impact of quercetin in the treatment of prostate cancer. Biomedicine & Pharmacotherapy. 2021; 138: 111548.

[2] Chhikara BS, Parang K. Global cancer statistics 2022: the trends projection analysis. Chemical Biology Letters. 2023; 10: 451.

[3] Haberkorn U, Eder M, Kopka K, Babich JW, Eisenhut M. New strategies in prostate cancer: prostate-specific membrane antigen (PSMA) ligands for diagnosis and therapy. Clinical Cancer Research. 2016; 22: 9–15.

[4] Xia C, Dong X, Li H, Cao M, Sun D, He S, et al. Cancer statistics in China and United States, 2022: profiles, trends, and determinants. Chinese Medical Journal. 2022; 135: 584–590.

[5] Liu L, Liu T, Tao W, Liao N, Yan Q, Li L, et al. Flavonoids from Scutellaria barbata D. Don exert antitumor activity in colorectal cancer through inhibited autophagy and promoted apoptosis via ATF4/sestrin2 pathway. Phytomedicine. 2022; 99: 154007.

[6] Chen Q, Rahman K, Wang S, Zhou S, Zhang H. Scutellaria barbata: a review on chemical constituents, pharmacological activities and clinical applications. Current Pharmaceutical Design. 2020; 26: 160–175.

[7] Cheng J, Guo Q, Wu X, MA S, Gao Y, Ya-Zhen S. Scutellaria barbata flavonoids improve the composited aβ-induced abnormal changes in glial cells of the brains of rats. Combinatorial Chemistry & High Throughput Screening. 2021; 25: 64–76.

[8] Shi H, Liu J, Fan J, He L, Wang X, Tang F, et al. Molecular assessment of scutellaria barbata D. Don in the treatment of nasopharyngeal carcinoma based on network pharmacology and experimental verification. Evidence-Based Complementary and Alternative Medicine. 2022; 2022: 1–13.

[9] Xue S, Geng A, Lian T, Liu Y. Scutellaria barbata D. Don inhibits cervical cancer cell proliferation, migration, and invasion via miR-195-5p/LOXL2 axis. Toxicology Research. 2022; 11: 804–811.

[10] Lin J, Feng J, Yang H, Yan Z, Li Q, Wei L, et al. Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway. Oncology Reports. 2017; 38: 2293–2300.

[11] Kretschmer A, Tilki D. Biomarkers in prostate cancer—current clinical utility and future perspectives. Critical Reviews in Oncology/Hematology. 2017; 120: 180–193.

[12] Sun P, Wang A, Zhang Z, Zhang Y, Zheng X. Network pharmacology-based strategy to investigate the active ingredients and molecular mechanisms of scutellaria barbata D. Don against radiation pneumonitis. Medicine. 2021; 100: e27957.

[13] Wang M, Chen Y, Hu P, Ji J, Li X, Chen J. Neoclerodane diterpenoids from Scutellaria barbata with cytotoxic activities. Natural Product Research. 2020; 34: 1345–1351.

[14] Mai C, Chin K, Foong L, Pang K, Yu B, Shu Y, et al. Modeling prostate cancer: what does it take to build an ideal tumor model? Cancer Letters. 2022; 543: 215794.

[15] Ritch C, Cookson M. Recent trends in the management of advanced prostate cancer. F1000Research. 2018; 7: 1513.

[16] Schatten H. Brief overview of prostate cancer statistics, grading, diagnosis and treatment strategies. Advances in Experimental Medicine and Biology. 2018; 74: 1–14.

[17] Sha S, Ni L, Stefil M, Dixon M, Mouraviev V. The human gastrointestinal microbiota and prostate cancer development and treatment. Investigative and Clinical Urology. 2020; 61: S43–S50.

[18] Yang P, Chen T, Zhao W, Liu G, Feng X, Wang S, et al. Scutellaria barbata D.Don and Oldenlandia diffusa (Willd.) Roxb crude extracts inhibit hepatitis-B-virus-associated hepatocellular carcinoma growth through regulating circRNA expression. Journal of Ethnopharmacology. 2021; 275: 114110.

[19] Jin Y, Chen W, Yang H, Yan Z, Lai Z, Feng J, et al. Scutellaria barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF-β/Smad signaling pathways. Experimental and therapeutic medicine. 2017; 14: 5527–5534.

[20] Kan X, Zhang W, You R, Niu Y, Guo J, Xue J. Scutellaria barbata D. Don extract inhibits the tumor growth through down-regulating of Treg cells and manipulating Th1/Th17 immune response in hepatoma H22-bearing mice. BMC Complementary and Alternative Medicine. 2017; 17: 41.

[21] Li H, Su J, Jiang J, Li Y, Gan Z, Ding Y, et al. Characterization of polysaccharide from Scutellaria barbata and its antagonistic effect on the migration and invasion of HT-29 colorectal cancer cells induced by TGF-β1. International Journal of Biological Macromolecules. 2019; 131: 886–895.

[22] Li L, Xu X, Wu L, Zhu H, He Z, Zhang B, et al. Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22-bearing mice. Molecular medicine reports. 2019; 19: 2254–2262.

[23] Ma T, Zhang G, Dai C, Zhang B, Cao K, Wang C, et al. Scutellaria barbata and Hedyotis diffusa herb pair for breast cancer treatment: potential mechanism based on network pharmacology. Journal of Ethnopharmacology. 2020; 259: 112929.

[24] Sheng D, Zhao B, Zhu W, Wang T, Peng Y. Scutellaria barbata D.Don (SBD) extracts suppressed tumor growth, metastasis and angiogenesis in Prostate cancer via PI3K/Akt pathway. BMC Complementary Medicine and Therapies. 2022; 22: 120.

[25] Wang S, Long S, Deng Z, Wu W. Positive role of Chinese herbal medicine in cancer immune regulation. The American Journal of Chinese Medicine. 2020; 48: 1577–1592.

[26] Wei L, Lin J, Xu W, Cai Q, Shen A, Hong Z, et al. Scutellaria barbata D. Don inhibits tumor angiogenesis via suppression of Hedgehog pathway in a mouse model of colorectal cancer. International Journal of Molecular Sciences. 2012; 13: 9419–9430.

[27] Xu X, Chen F, Zhang L, Liu L, Zhang C, Zhang Z, et al. Exploring the mechanisms of anti-ovarian cancer of Hedyotis diffusa Willd and Scutellaria barbata D. Don through focal adhesion pathway. Journal of Ethnopharmacology. 2021; 279: 114343.

[28] Yang P, Xu P, Cheng C, Jiao J, Wu Y, Dong S, et al. Integrating network pharmacology and experimental models to investigate the efficacy of QYHJ on pancreatic cancer. Journal of Ethnopharmacology. 2022; 297: 115516.

[29] Murillo-Garzón V, Kypta R. WNT signalling in prostate cancer. Nature Reviews Urology. 2017; 14: 683–696.

[30] Yin X, Zhou J, Jie C, Xing D, Zhang Y. Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line a549. Life Sciences. 2004; 75: 2233–2244.

[31] Yue GG, Chan YY, Liu W, Gao S, Wong CW, Lee JK, et al. Effectiveness of Scutellaria barbata water extract on inhibiting colon tumor growth and metastasis in tumor-bearing mice. Phytotherapy Research. 2021; 35: 361–373.

[32] Zhang L, Ren B, Zhang J, Liu L, Liu J, Jiang G, et al. Anti-tumor effect of Scutellaria barbata D. Don extracts on ovarian cancer and its phytochemicals characterisation. Journal of Ethnopharmacology. 2017; 206: 184–192.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Directory of Open Access Journals (DOAJ) DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online for everyone.

SCImago The SCImago Journal & Country Rank is a publicly available portal that includes the journals and country scientific indicators developed from the information contained in the Scopus® database (Elsevier B.V.)

Publication Forum - JUFO (Federation of Finnish Learned Societies) Publication Forum is a classification of publication channels created by the Finnish scientific community to support the quality assessment of academic research.

Scopus: CiteScore 0.9 (2023) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Norwegian Register for Scientific Journals, Series and Publishers Search for publication channels (journals, series and publishers) in the Norwegian Register for Scientific Journals, Series and Publishers to see if they are considered as scientific. (https://kanalregister.hkdir.no/publiseringskanaler/Forside).

Submission Turnaround Time

Conferences

Top