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Original Research

Open Access

Expression and immunolocalisation of PPARγ in reproductive tissues of rabbits fed n-3 PUFA-enriched diets

  • Sofia Passaponti1,†
  • Cinzia Signorini2,*,
  • Giulia Collodel2
  • Elena Moretti2
  • Simona Mattioli3
  • Giuseppe Belmonte2
  • Daria Noto2,†

1Department of Life Sciences, University of Siena, 53100 Siena, Italy

2Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy

3Department of Agricultural, Environmental, and Food Science, University of Perugia, 06123 Perugia, Italy

DOI: 10.31083/j.jomh1803072 Vol.18,Issue 3,March 2022 pp.1-8

Submitted: 26 November 2021 Accepted: 17 January 2022

Published: 31 March 2022

*Corresponding Author(s): Cinzia Signorini E-mail: cinzia.signorini@unisi.it

† These authors contributed equally.

Abstract

Background: Since fatty acids are natural ligands of peroxisome proliferator-activated receptors (PPARs), involved in a wide spectrum of metabolic activities, we explored the protein expression and localisation of PPARγ, as well as susceptibility to lipoperoxidation (LPO), in reproductive tissues of rabbits fed n-3 polyunsaturated fatty acid (PUFA)-enriched diets. Methods: Nine rabbit bucks were divided into three groups and fed different diets: FLAX diet supplemented with 10% extruded flaxseed, FISH diet containing 3.5% fish oil and standard diet as control. Protein expression by western blot analysis, localisation of PPARγ by immunofluorescence and susceptibility to LPO by measuring F2-Isoprostanes (F2-IsoPs) were explored. Results: Down-regulation of PPARγ expression was observed in control rabbit testes compared with FLAX (p < 0.01) and FISH (p < 0.05) dietary groups. On the contrary, an up-regulation in rabbit epididymis was observed in both n-3 PUFAs dietary groups compared with the control (p < 0.001). Immunofluorescent staining of PPARγ showed a strong expression in the interstitial tissue and seminiferous tubules of testes from groups fed n-3 PUFA-enriched diets compared to controls; the PPARγ signal was brighter in the epididymis of controls than in those of rabbits fed n-3 PUFA-enriched diets. Interestingly, F2-IsoP levels were significantly higher in testis and epididymal tissue of control compared to n-3 PUFAs dietary groups (p < 0.001). Conclusion: PPARγ is present in male reproductive tissues, in particular in the epididymis and interstitial testicular tissue. PPARγ expression appears to be influenced by n-3 PUFAs dietary sources and to play a role in supporting sperm maturation. n-3 PUFAs diets, reducing F2-IsoP levels, commonly considered proinflammatory molecules linked to oxidative injuries, may also support PPARγ activity.


Keywords

Peroxisome proliferator-activated receptors; Polyunsaturated fatty acids; N-3 fatty acid; Diet; Reproductive tissues; Im-munofluorescence; Western blot; F2-isoprostanes; Animal model

Cite and Share

Sofia Passaponti,Cinzia Signorini,Giulia Collodel,Elena Moretti,Simona Mattioli,Giuseppe Belmonte,Daria Noto. Expression and immunolocalisation of PPARγ in reproductive tissues of rabbits fed n-3 PUFA-enriched diets. Journal of Men's Health. 2022. 18(3);1-8.

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