Title
Author
DOI
Article Type
Special Issue
Volume
Issue
Assessment of male football players' physical fitness levels based on certain gene (AGT rs699 & IL-6 rs1800795) polymorphisms
1Faculty of Sports Science, Bingol University, 12000 Bingol, Türkiye
2Laboratory for Industrial and Applied Mathematics (LIAM), Department of Mathematics and Statistics, York University, Toronto, ON M3J1P3, Canada
3Human Nutrition Unit (HNU), Department of Food and Drugs, Medical School, University of Parma, 43125 Parma, Italy
4School of Physical Education and Sports, Osmaniye Korkut Ata University, 80000 Osmaniye, Türkiye
5Faculty of Sports Science, Gazi University, 06500 Ankara, Türkiye
6Faculty of Sports Science, Lokman Hekim University, 06530 Ankara, Türkiye
7Department of Basic Medical Sciences, Marmara University, 34854 İstanbul, Türkiye
8Department of Medical Biology and Genetics, Marmara University, 34722 Istanbul, Türkiye
9Physical Education and Sports Teaching Department, Kazim Karabekir Faculty of Education, Ataturk University, 25240 Erzurum, Türkiye
10Faculty of Physical Culture and Health, University in Tetovo, 1200 Tetova, Republic of North Macedonia
DOI: 10.22514/jomh.2025.050 Vol.21,Issue 4,April 2025 pp.34-45
Submitted: 08 November 2023 Accepted: 04 December 2023
Published: 30 April 2025
*Corresponding Author(s): Nicola L. Bragazzi E-mail: nicolaluigi.bragazzi@unipr.it
*Corresponding Author(s): Halil İbrahim Ceylan E-mail: halil.ceylan@atauni.edu.tr
Background: The present investigation aims to elucidate the physical fitness attributes inherent in male football players about the Angiotensinogen (AGT) rs699 and Interleukin-6 (IL-6) rs1800795 gene polymorphisms. Methods: Twenty-two male football players, aged 18 to 35 years, voluntarily enrolled in the study conducted within the North Macedonian Super League. Genomic DNA was extracted from oral epithelial cells. Genotyping procedures were then executed using real-time polymerase chain reaction (RT-PCR). All participants were actively involved in an intensive training program six days a week throughout the six-week pre-season preparation phase. The male football players underwent physical assessments both before and after the training program. Statistical analysis involved the use of the Paired-Sample t-Test to discern differences between the pre-test and post-test measurements of the male football players. Results: When stratifying the outcomes according to the IL-6 genotype and AGT genotype variables, statistically significant differences were not observed in Squat Jump (SJ), 5 m sprint, 30 m sprint, Counter Movement Jump (CMJ), Drop Jump (DJ) evaluations, and body fat percentage (p > 0.05). In contrast, statistically significant differences were observed in the Yo-Yo Intermittent Recovery Test Level 2 (Yo-Yo IRT 2), 10 m sprint, and One Repetition Maximum (1RM) bench press variables (Yo-Yo IRT 2: CC and CT p = 0.005, <0.001; 10 m sprint: CT p = 0.024; and 1RM bench press: CC, CT and TT p < 0.001, <0.001, 0.045, respectively). Significant differences were also identified in the Yo-Yo IRT 2, 10 m sprint, and 1RM bench press measurements (Yo-Yo IRT 2: CC, CG and GG p = 0.002, 0.021, 0.001; 10 m sprint: CC and GG p = 0.020, 0.028; and 1RM bench press: CC, GG p = 0.001, 0.001, respectively). Conclusions: In summary, the AGT rs699 and IL-6 rs1800795 gene polymorphisms may play a role in the adaptations induced by training in male football players.
Football; Physical fitness; Sprint; Jump ability; Strength; AGT; IL-6
Celal Bulgay,Nicola L. Bragazzi,Orhan Gök,Veli O. Çakır,Selin Y. Tuncer,Tolga Polat,Korkut Ulucan,Halil İbrahim Ceylan,Metin Dalip,Mehmet S. Kocak,Mesut Cerit. Assessment of male football players' physical fitness levels based on certain gene (AGT rs699 & IL-6 rs1800795) polymorphisms. Journal of Men's Health. 2025. 21(4);34-45.
[1] Semenova EA, Hall ECR, Ahmetov II. Genes and athletic performance: the 2023 update. Genes. 2023; 14: 1235.
[2] Furrer R, Hawley JA, Handschin C. Themolecular athlete: exercise physiology frommechanisms to medals. Physiological Reviews. 2023; 103: 1693–1787.
[3] Leońska-Duniec A, Borczyk M, Piechota M, Korostyński M, Brodkiewicz A, Cięszczyk P. TTN variants are associated with physical performance and provide potential markers for sport-related phenotypes. International Journal of Environmental Research and Public Health. 2022; 19: 10173.
[4] Wackerhage H, Miah A, Harris RC, Montgomery HE, Williams AG. Genetic research and testing in sport and exercise science: a review of the issues. Taylor & Francis. 2009; 27: 1109–1116.
[5] Bulgay C, Kasakolu A, Kazan HH, Mijaica R, Zorba E, Akman O, et al. Exome-wide association study of competitive performance in elite athletes. Genes. 2023; 14: 660.
[6] Hudson AL, Gandevia SC, Butler JE. A principle of neuromechanical matching for motor unit recruitment in human movement. Exercise and Sport Sciences Reviews. 2019; 47: 157–168.
[7] Kumagai H, Kaneko T, Shintake Y, Miyamoto-Mikami E, Tomita H, Fukuo M, et al. Genetic polymorphisms related to muscular strength and flexibility are associated with artistic gymnastic performance in the Japanese population. European Journal of Sport Science. 2023; 23: 955–963.
[8] Konopka MJ, van den Bunder JCML, Rietjens G, Sperlich B, Zeegers MP. Genetics of long-distance runners and road cyclists—a systematic review with meta-analysis. Scandinavian Journal of Medicine and Science in Sports. 2022; 32: 1414–1429.
[9] Bouchard C, Rankinen T, Timmons JA. Genomics and genetics in the biology of adaptation to exercise. Comprehensive Physiology. 2011; 1: 1603–1648.
[10] Varillas-Delgado D, Del Coso J, Gutiérrez-Hellín J, Aguilar-Navarro M, Muñoz A, Maestro A, et al. Genetics and sports performance: the present and future in the identification of talent for sports based on DNA testing. European Journal of Applied Physiology. 2022; 122: 1811–1830.
[11] Guth LM, Roth SM. Genetic influence on athletic performance. Current Opinion in Pediatrics. 2013; 25: 653–658.
[12] Mann TN, Lamberts RP, Lambert MI. High responders and low responders: factors associated with individual variation in response to standardized training. Sports Medicine. 2014; 44: 1113–1124.
[13] Ahmetov II, Hall ECR, Semenova EA, Pranckevičienė E, Ginevičienė V. Advances in sports genomics. Advances in Clinical Chemistry. 2022; 107: 215–263
[14] Gomez-Gallego F, Santiago C, González-Freire M, Yvert T, Muniesa CA, Serratosa L, et al. The C allele of the AGT Met235Thr polymorphism is associated with power sports performance. Applied Physiology, Nutrition, and Metabolism. 2009; 34: 1108–1111.
[15] Aleksandra Z, Zbigniew J, Waldemar M, Agata LD, Mariusz K, Marek S, et al. The AGT gene M235T polymorphism and response of power-related variables to aerobic training. Journal of Sports Science and Medicine. 2016; 15: 616–624.
[16] De Cavanagh EMV, Inserra F, Ferder L. Angiotensin II blockade: a strategy to slow ageing by protecting mitochondria? Cardiovascular Research. 2011; 89: 31–40.
[17] Zhuo JL. Augmented intratubular renin and prorenin expression in the medullary collecting ducts of the kidney as a novel mechanism of angiotensin II-induced hypertension. American Journal of Physiology-Renal Physiology. 2011; 301: F1193–1194.
[18] Zarębska A, Sawczyn S, Kaczmarczyk M, Ficek K, Maciejewska-Karłowska A, Sawczuk M, et al. Association of rs699 (m235t) polymorphism in the agt gene with power but not endurance athlete status. The Journal of Strength & Conditioning Research. 2013; 27: 2898–2903.
[19] Aleksandra Z, Zbigniew J, Waldemar M, Agata LD, Mariusz K, Marek S, et al. The AGT gene M235T polymorphism and response of power-related variables to aerobic training. Journal of Sports Science and Medicine. 2016; 15: 616–624.
[20] Southward K, Rutherfurd-Markwick K, Badenhorst C, Ali A. The role of genetics in moderating the inter-individual differences in the ergogenicity of caffeine. Nutrients. 2018; 10: 1352.
[21] Eider J, Cieszczyk P, Leońska-Duniec A, Maciejewska A, Sawczuk M, Ficek K, et al. Association of the 174 G/C polymorphism of the IL6 gene in polish power-orientated athletes. The Journal of Sports Medicine and Physical Fitness. 2013; 53: 88–92.
[22] Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014; 6: a016295.
[23] Kotowska J, Jówko E, Cieśliński I, Gromisz W, Sadowski J. IL-6 and HSPA1A gene polymorphisms may influence the levels of the inflammatory and oxidative stress parameters and their response to a chronic swimming training. International Journal of Environmental Research and Public Health. 2022; 19: 8127.
[24] MacDonald C, Wojtaszewski JF, Pedersen BK, Kiens B, Richter EA. Interleukin-6 release from human skeletal muscle during exercise: relation to AMPK activity. Journal of Applied Physiology. 2003; 95: 2273–2277.
[25] Robson-Ansley PJ, de Milander L, Collins M, Noakes TD. Acute interleukin-6 administration impairs athletic performance in healthy, trained male runners. Canadian Journal of Applied Physiology. 2004; 29: 411–418.
[26] González-Estrada GD, Berrio GB, Gómez-Ríos D. Association between ACE, ACTN3, AGT, BDKRB2, and IL-6 gene polymorphisms and elite status in Colombian athletes. Journal of Physical Education and Sport. 2023; 23: 1036–1043.
[27] Mandic R, Jakovljevic S, Jaric S. Effects of countermovement depth on kinematic and kinetic patterns of maximum vertical jumps. Journal of Electromyography and Kinesiology. 2015; 25: 265–272.
[28] Mayhew SR, Wenger HA. Time motion analysis of professional soccer. Journal of Human Movement Studies. 1985; 11: 49–52.
[29] ACSM. American College of Sports Medicine-Guidelines for exercise testing and prescription. 11th edn. ACSM: USA. 2021.
[30] Edward TH, Dixie LT. Fitness professional’s handbook. 6th edn. Human Kinetics: USA. 2012.
[31] Jackson AS, Pollock ML. Generalized equations for predicting body density of men. British Journal of Nutrition. 1978; 40: 497–504.
[32] Cohen J. Statistical power analysis for the behavioral sciences. 2nd edn. Erbbaum: Hillsdale, NJ. 1988.
[33] Kline RB. Principles and practice of structural equation modeling. 3rd edn. Guilford Press: New York, NY. 2011.
[34] Hopkins WG, Marshall SW, Batterham AM, Hanin J. Progressive statistics for studies in sports medicine and exercise science. Medicine & Science in Sports & Exercise. 2009; 41: 3.
[35] Demirci B, Bulgay C, Ceylan Hİ, Öztürk ME, Öztürk D, Kazan HH, et al. Association of ACTN3 R577X polymorphism with elite basketball player status and training responses. Genes. 2023; 14: 1190.
[36] Eroğlu O, Zileli R. The effect of genetic factors on sportive performance. International Journal of Sports, Exercise & Training Science. 2015; 1: 63–76.
[37] Grgic J. Effects of caffeine on resistance exercise: a review of recent research. Sports Medicine. 2021; 51: 2281–2298.
[38] McAuley ABT, Hughes DC, Tsaprouni LG, Varley I, Suraci B, Roos TR, et al. Genetic association research in football: a systematic review. European Journal of Sport Science. 2021; 21: 714–752.
[39] Massidda M, Voisin S, Culigioni C, Piras F, Cugia P, Yan X, et al. ACTN3 R577X polymorphism is associated with the incidence and severity of injuries in professional football players. Clinical Journal of Sport Medicine. 2019; 29: 57–61.
[40] Massidda M, Bachis V, Corrias L, Piras F, Scorcu M, Culigioni C, et al. ACTN3 R577X polymorphism is not associated with team sport athletic status in Italians. Sports Medicine-Open. 2015; 1: 6.
[41] Varillas-Delgado D, Gutierrez-Hellin J, Maestro A. Genetic profile in genes associated with sports injuries in elite endurance athletes. International Journal of Sports Medicine. 2023; 44: 64–71.
[42] Maciejewska-Skrendo A, Sawczuk M, Cieszczyk P, Ahmetov I. Genes and power athlete status: genes and power athlete status. In Barh D, Ahmetov I (eds.) Sports, exercise, and nutritional genomics (pp. 41–72). Academic press: Cambridge, UK. 2019.
[43] González-Estrada GD, Berrio GB, Gómez-Ríos D. Association between ACE, ACTN3, AGT, BDKRB2, and IL-6 gene polymorphisms and elite status in Colombian athletes. Journal of Physical Education and Sport. 2023; 23: 1036–1043.
[44] Ben-Zaken S, Meckel Y, Nemet D, Pantanowitz M, Eliakim A. The AGT M235T (RS699, 4072T>C) polymorphism is not associated with elite weightlifting performance. Acta Kinesiologiae Universitatis Tartuensis. 2018; 23: 34.
[45] Gomez-Gallego F, Santiago C, González-Freire M, Yvert T, Muniesa CA, Serratosa L, et al. The C allele of the AGT Met235Thr polymorphism is associated with power sports performance. Applied Physiology, Nutrition and Metabolism. 2009; 34: 1108–1111.
[46] Ruiz JR, Arteta D, Buxens A, Artieda M, Gómez-Gallego F, Santiago C, et al. Can we identify a power-oriented polygenic profile? Journal of Applied Physiology. 2010; 108: 561–566.
[47] Bulgay C, Polat T, Yılmaz OÖ, Tacal Aslanet B, Ali Ergün M, Ulucan K. Determination of Angiotensinogen (AGT) rs699 Polymorphism Distribution in Football Players. Kilis 7 Aralık University Journal of Physical Education and Sports Sciences. 2021; 5: 145–153.
[48] Eynon N, Ruiz JR, Oliveira J, Duarte JA, Birk R, Lucia A. Genes and elite athletes: a roadmap for future research. Journal of Physiology. 2011; 589: 3063–3070.
[49] Tuna G, Polat T, Yılmaz ÖÖ, Savaşan M, Erdil NG, Ulucan K, et al. The relationship between swimming styles and Il-6 Rs1800795 polymorphism in professional swimmers. Pakistan Journal of Medical & Health Sciences. 2022; 16: 444–445.
[50] Ulucan K, Yuksel I, Doğan CS, Kaynaret Ö. Interleukin-6 rs1800795 polymorphism is not considered as a genetic biomarker in Turkish national skiing running athlete cohort. Fresenius Environ Bull. 2020; 3: 1–5.
[51] Cenikli A, Nursal A, Tural E, Polat Y, Tasmektepligil MY, Yigit S. The correlation between rs1800795 variant of IL-6 and sports performance among Turkish elite athletes. International Journal of Humanities Social Sciences and Education. 2016; 1: 3–11.
[52] Huuskonen A, Tanskanen M, Lappalainen J, Oksala N, Kyröläinen H, Atalay M. A common variation in the promoter region of interleukin-6 gene shows association with exercise performance. Journal of Sports Science and Medicine. 2009; 8: 271–277.
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